Dramatic increase in efficiency and speed of regeneration
Proven in many different species, and in recalcitrant genotypes
Wide use in plant tissue culture, regeneration, gene editing, transformation, propagation etc
Researchers at the Institute of Developmental Genetics and Biology, Chinese Academy of Sciences, Beijing have discovered a natural plant peptide that strongly promotes plant regeneration in a wide range of species. The factor, REF1 (REGENERATION FACTOR1) is stable and, when applied exogenously in culture medium, dramatically increases regeneration efficiency. This brings associated enhancements in, for example, transformation efficiency. Inclusion in culture medium, or other form of exogenous application, is once-off and it does not need to be continuously supplied to gain an effect. In addition, REF1 dramatically accelerates the tissue culture/regeneration process. Use of REF1 typically cuts 10-20 days off the time to recover transplantable seedlings from culture (e.g. 40 days for soybean, compared to 60 days without REF1). Plants recovered are all whole, viable and fertile.
The REF1 regeneration enhancement technology (21.691) is available through PBL. Further information can be obtained under confidentiality agreement.
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