Tech ID: 21.689
The inventors observed that short “paperclip” RNAs (pcRNAs), with partially closed ends, efficiently enter cells via a clathrin-independent pathway, are more stable than other forms of RNA and effectively facilitate transcript knockdown. This alternative RNA structure provides a novel approach to control insect pests that either are recalcitrant to RNAi or where resistance to RNAi-insecticides has arisen through changes in dsRNA uptake mechanisms.
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Published: WO 2022/011452
Roohollah Abbasi, Daniel Heschuk, Brandon Kim, Steve Whyard (2020). A novel paperclip double-stranded RNA structure demonstrates clathrin-independent uptake in the mosquito Aedes aegypti. Insect Biochemistry and Molecular Biology: 127. https://doi.org/10.1016/j.ibmb.2020.103492