Root-specific Promoters

Tech ID: 06.414

Key Features

• Promoters of genes involved in oat avenacin biosynthesis with root-specific expression profiles
• Expression profile tested in monocot and dicot plant species demonstrating the use for tissue-selective expression of genes in transgenic crops
• Potential use for expression of genes in monocot and dicot crop species
• Sad1 and Sad2 promoters drive root-specific expression in wheat

Description

The researchers have identified two promoters involved in the expression of different avenacin biosynthesis genes in the roots of oats. Reporter fusion experiments have demonstrated the potential use of these promoters for root-specific expression of heterologous genes in both monocot (rice) and dicot (Arabidopsis) crop species, with similar expression profiles to those shown for their native genes in oats. Update: Sad1 and Sad2 promoters drive root-specific expression in wheat.

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Patents

Granted: US 7,982,096

References

Qi X et al (2006).  A different function for a member of an ancient and highly conserved cytochrome P450 family: From essential sterols to plant defense.  PNAS; 103(49): 18848-18853; https://doi.org/10.1073/pnas.0607849103

Qi X et al (2004).  A gene cluster for secondary metabolism in oat: Implications for the evolution of metabolic diversity in plants.  PNAS; 101(21): 8233-8238.  https://doi.org/10.1073/pnas.0401301101

Haralampidis K et al (2002).  Biosynthesis of triterpenoid saponins in plants.  Adv Biochem Eng Biotechnol; 75: 31-49.  https://doi.org/10.1007/3-540-44604-4_2

Haralampidis K et al (2001).  A new class of oxidosqualene cyclases directs synthesis of antimicrobial phytoprotectants in monocots.  PNAS; 98(23): 13431-13436.  https://doi.org/10.1073/pnas.231324698