Tech ID: 99.194
Efficient methods for expression of genes of interest in plants are required for many applications. Existing methods require the use of either transgenic plants (which are time and resource consuming) or inefficient transient expression systems. By combining suppressors of gene silencing with Agrobacterium infiltration-mediated transient expression, a rapid and highly efficient method for gene expression in plants has been developed.
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Published: WO 01/038512
Granted: EP 1232274; US 7,217,854; US 8,871,997; CA 2,390,152; ZA 20023388; AU 782788; IL 149388; JP 4884628
Olivier Voinnet et al (2003). An enhanced transient expression system in plants based on suppression of gene silencing by the P19 protein of tomato bushy stunt virus. The Plant Journal; 33(5): 949-956. https://doi.org/10.1046/j.1365-313X.2003.01676.x
Olivier Voinnet et al (1999). Suppression of gene silencing: a general strategy used in diverse DNA and RNA viruses of plants. PNAS; 96(24): 14147-14152.