siRNA for Gene Silencing

Tech ID: 99.190

Key Features

  • Gene Silencing: siRNA technology for Plants and other Organisms
  • Detecting the presence of gene silencing
  • Inducing gene silencing

Description

RNA interference or RNAi is rapidly becoming of great importance to mammalian cell research including research on human cells. Professor David Baulcombe and colleagues at the Sainsbury Laboratory, Norwich, UK, were the first to identify a species of target sequence specific small RNAs of between 21 and 25 nucleotides as being diagnostic of RNAi in a range of organisms, including plants. Detection of these short RNAs can be used as a diagnostic tool for correlating the efficiency of silencing of a target gene in any experimental system including the use of RNAi. More importantly siRNAs can also be used to effect gene silencing by introducing siRNAs into organisms to silence specific target genes.

For further detailed information please download the non-confidential summary pdf.

Patents

Granted: US 6,753,139; US 7,704,688; US 8,097,710; US 8,258,285; US 8,263,569; US 8,299,235; US 8,349,607

References

AJ Hamilton and DC Baulcombe (1999).  A species of small antisense RNA in posttranscriptional gene silencing in plants.  Science; 286: 950-952.  https://doi.org/10.1126/science.286.5441.950

Contact: Dr Lars von Borcke

Inventors
David Baulcombe and Andrew Hamilton
The Sainsbury Laboratory (Norwich, UK)