TRV-based Gene Silencing

Tech ID: 04.365

Key Features

• Novel Tobacco Rattle Virus VIGS Vectors
• Improvements to PBL’s gene function analysis methods in plants
• Efficient gene silencing system
• Rapid means of gaining insight into gene function in plants

Description

The TRV VIGS technology originally developed by Professor Sir David Baulcombe while at the Sainsbury Laboratory, is now complemented by new, modified vectors and modifications building on the strength of the TRV VIGS system for studying gene function in plants. The modified TRV vectors and methods, developed by Dinesh-Kumar at Yale, deliver the following additional benefits to the existing PBL TRV technology, more efficient silencing (including broader demonstration in more plant species), more efficient production of viral RNA and a multiple cloning site for easy insertion of sequences.

For further detailed information please download the non-confidential summary pdf.

Patents

Granted: US 7,229,829

References

Burch-Smith TM et al (2004).  Applications and advantages of virus induced gene silencing (VIGS) for gene function studies in plants.  Plant Journal; 39(5): 734-746.  https://doi.org/10.1111/j.1365-313x.2004.02158.x

Liu Y et al (2002).  Virus-induced gene silencing in tomato.  Plant Journal; 31(6): 777-786.  https://doi.org/10.1046/j.1365-313X.2002.01394.x

Liu Y et al (2002).  Tobacco rar1, eds1 and npr1/nim1 like genes are required for N-mediated resistance to tobacco mosaic virus.  Plant Journal; 30(4): 415-429.  https://doi.org/10.1046/j.1365-313X.2002.01297.x