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Single peptides / reporter genes

Initially work focussed on reporter genes like GUS, the diagram below demonstrates the difference in expression levels from the wild type CPMV vector compared with the CPMV-HT protein expression system, boosting yields 15 fold


Single peptides (as the DsRed in the example below) have been produced with the CPMV-HT system with yields of up to 1.5g of protein per 1 kg of leaf tissue.

Above on the left is the infected leaf, in the middle the extract (left tube control) and on the right hand side the protein gel from the extract showing the DsRed indicted by the red arrow.



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